annexin v fitc apoptosis detection kit  (Elabscience Biotechnology)

Journal: Advanced Science

Article Title: Phosphorylated STYK1 Promotes Gemcitabine Resistance via Autophagy‐Mediated Cancer Stemness in Pancreatic Cancer

doi: 10.1002/advs.202521488

Figure Lengend Snippet: STYK1 promotes gemcitabine resistance in pancreatic cancer cells. (A, B) PANC‐1 cells were treated with different concentrations of gemcitabine for the indicated time periods, and STYK1 mRNA expression was assessed by RT‐qPCR (n = 6). **** p < 0.0001 according to Student's t ‐test. (C) Western blotting analysis of STYK1 expression levels in human pancreatic cancer cell lines PANC‐1 and AsPC‐1, as well as in the gemcitabine‐resistant cell lines PANC‐1/GEM and AsPC‐1/GEM. D) RT‐PCR analysis of STYK1 mRNA levels in PANC‐1, AsPC‐1, PANC‐1/GEM and AsPC‐1/GEM cells. **** p < 0.0001 according to Student's t ‐test. (E) Representative images of IHC staining for STYK1 in gemcitabine‐sensitive and resistant pancreatic cancer PDX tumors tissues. (F–I) Cell viability of PANC‐1/GEM and AsPC‐1/GEM cells with stable STYK1 knockdown, as well as PANC‐1 and AsPC‐1 cells stably overexpressing STYK1, following treatment with different concentrations of gemcitabine for 72 h, as determined by CCK‐8 assay. (J) PANC‐1/GEM cells with stable STYK1 knockdown were treated with gemcitabine (5 µM) for different durations as indicated, and OD values were assessed using the CCK‐8 assay. **** p < 0.0001 according to Student's t ‐test. (K–M) Apoptosis of PANC‐1/GEM and AsPC‐1/GEM cells with stable STYK1 knockdown following gemcitabine treatment (5 µM for PANC‐1/GEM and 40 µM for AsPC‐1/GEM, 24 h), assessed using an Annexin V‐AbFluor 488/PI double staining Apoptosis Detection Kit. Annexin V‐positive cells indicate early apoptosis, whereas PI‐positive cells indicate late apoptosis/necrosis (n = 3). **** p < 0.0001 according to Student's t ‐test. (N) Representative images of resected tumors from nude mice in different groups (n = 5). O) Tumor weights of resected tumors in different groups of mice (n = 5). **** p < 0.0001 according to Student's t ‐test. ** p < 0.01; **** p < 0.0001 according to Student's t ‐test. (P) Tumor volume change curves in different groups of mice (n = 5); Tumor volume was measured every 5 days after cell injection and during gemcitabine treatment (25 mg/kg). **** p < 0.0001 according to Student's t ‐test. (Q) Representative H&E staining and Ki67 immunohistochemical staining images of resected tumor tissues (n = 5). Scale bar: 50 µm. Data were expressed as mean ± SD, * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 according to Student's t ‐test.

Article Snippet: Apoptosis was assessed using an Annexin V‐FITC Apoptosis Detection Kit (Elabscience, E‐CK‐A252).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Analysis, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Knockdown, Stable Transfection, CCK-8 Assay, Double Staining, Injection, Staining, Immunohistochemical staining

Journal: Blood Cancer Journal

Article Title: The role of RAD23A in DNA repair and proteasome-dependent protein degradation in multiple myeloma

doi: 10.1038/s41408-026-01574-z

Figure Lengend Snippet: A Proteomic-based GSEA Reactome showing negative enrichment of the deubiquitination pathway following RAD23A knockout (NES = −1.398, FDR q -value = 0.104). B MM.1R and AMO1 RAD23A-iKO cells, as well as H929, ANBL6-WT, and ANBL6-BR RAD23A-iKD cells, were cultured with Dox as described in Fig. . Protein lysates were subjected to immunoblotting using antibodies against K48-linked polyubiquitin and β-actin. CT = control. C Aggresome and K48-linked polyubiquitinated protein (K48-polyU) accumulation were detected using an aggresome detection kit and anti-K48-polyubiquitin antibody, followed by confocal microscopy (60×). D MM.1R RAD23A-iKO cells were cultured with Dox for 9 days. MM.1R cells treated with DMSO or 1 µM bortezomib for 3 h were used as controls. Cell pellets were harvested and lysed. 20S proteasome activities (CTL, chymotrypsin-like; TL, trypsin-like; CL, caspase-like) were measured. Proteasome activity was normalized to the DMSO control or parental MM.1R control cells (mean ± SD, n = 3, assessed by two-way ANOVA with Tukey’s test). E MM.1R and AMO1 RAD23A-iKO cells, as well as H929, ANBL6-WT and ANBL6-BR RAD23A-iKD cells, were prepared as described in Fig. . Cells were lysed and immunoblotted using antibodies against p-eIF2α, ATF4, CHOP, PARP, cleaved caspase-3, and β-actin. F Caspase-3/7 activation was detected using the IncuCyte Caspase-3/7 Green Dye and live-cell imaging. G Apoptosis was analyzed by flow cytometry using Annexin V-FITC/DAPI double staining (mean ± SD, n = 3, assessed by two-way ANOVA with Sidak’s test).

Article Snippet: Apoptosis was further validated by flow cytometric analysis using an Annexin V-FITC/DAPI apoptosis detection kit (Elabscience, #E-CK-A252), following the manufacturer’s protocol.

Techniques: Knock-Out, Cell Culture, Western Blot, Control, Confocal, Microscopy, Activity Assay, Activation Assay, Live Cell Imaging, Flow Cytometry, Double Staining

Journal: BMC Complementary Medicine and Therapies

Article Title: In vitro analysis of the molecular mechanisms of ursolic acid against ovarian cancer

doi: 10.1186/s12906-025-04808-y

Figure Lengend Snippet: Ursolic acid promotes apoptosis in SKOV3 cells. Ursolic acid (50 µmol/L, 100 µmol/L) was used to treat SKOV3 cells for 24 h. (A) The cell apoptosis rate of transfected cells was determined by flow cytometry. (B) Evaluation of apoptotic cells by TUNEL staining in untreated and ursolic acid-treated SKOV3 cells. TUNEL (red) and DAPI (blue) were used as nuclear stains. The experiments were repeated three times. (C) The protein expression of Bax, Bcl-2, Caspase-3 and c-Caspase-3 in SKOV3 cells treated with various concentrations of UA was evaluated by Western blotting. β-Actin(actin beta) was used as a loading control. (D) The band densities were measured using ImageJ software to quantify the bands. The average was calculated from three independent experiments. (E-F) Molecular docking formula of Bax and Bcl-2. Compared to the control group, * p < 0.05, ** p < 0.01; compared to the cisplatin group, # p < 0.05, ## p < 0.01

Article Snippet: The Annexin V-conjugated FITC apoptosis detection kit was supplied by Elabscience.

Techniques: Transfection, Flow Cytometry, TUNEL Assay, Staining, Expressing, Western Blot, Control, Software

Journal: BMC Complementary Medicine and Therapies

Article Title: In vitro analysis of the molecular mechanisms of ursolic acid against ovarian cancer

doi: 10.1186/s12906-025-04808-y

Figure Lengend Snippet: Schematic illustration of how UA promotes endoplasmic reticulum stress, inhibits autophagy, and induces apoptosis (By Fig Draw)

Article Snippet: The Annexin V-conjugated FITC apoptosis detection kit was supplied by Elabscience.

Techniques: